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p pdk1  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc p pdk1
    P Pdk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+pdk1/PDK1+Antibody/pmc12990956-78-57-59
    Average 96 stars, based on 987 article reviews
    p pdk1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Minnelide exhibits antileukemic activity by targeting the Ars2/miR-190a-3p axis.
    Article Snippet: GAPDH (2118), AKT (4685S), p-AKT (Ser473, 4051S), p-PDK1 (Ser241, 3438S), Cyclin D (55506S), Dicer (5362S), Drosha (3364S), p27 Kip1 (3686S), Caspase 3 (9661S), PARP (9532S), Caspase 9 (9505S) and COX IV (4844S) were obtained from Cell Signaling Technology (Boston, USA).

    Membrane:

    Article Title: Identification of signaling networks associated with lactate modulation of macrophages and dendritic cells
    Article Snippet: The membranes were subsequently blocked overnight at 4 °C in 10 mL of tris-buffered saline with 0.1% (v/v) Tween-20 (TBST), supplemented with 5% (w/v) bovine serum albumin (Roche). .. The membrane was incubated with each primary antibody targeting phosphorylated STAT1 (p-STAT1, Tyr701, D4A7, Cell signaling technology (CST) #7649), p-STAT2 (Tyr690, CST #4441), p-STAT3 (Ser727, CST #9134), p-SAPK/JNK (Thr183/Tyr185, 81E11, CST #4668), p-SAPK/JNK (Thr183/Tyr185, 98F2, CST #4671), p-NF-κB p65 (Ser536, 93H1, CST #3033), β-Catenin (Ser45, D2U8Y, CST #19807), p-Akt (Ser473, CST #9271), p-Akt (Thr308, D25E6, CST #13038), p-Akt (Ser473, D9E, CST #4060), Akt (pan) (C67E7, CST #4691), p-PTEN (Ser380, CST #9551), p-GSK-3β (Ser9, D85E12, CST #5558), p-c-Raf (Ser259, CST #9421), p-PDK1 (Ser241, C49H2, CST #3438), p-p44/42 MAPK (Erk1/2, Thr202/Tyr204, D13.14.4E, CST #4370), p-p38 MAPK (Thr180/Tyr182, D3F9, CST #4511), and β-actin (CST #4967). .. The membrane was then washed with TBST, and a secondary antibody was incubated for 1 h. The membrane was then washed with TBST followed by chemiluminescence detection using HRP substrate (SuperSignal West Pico/Femto Chemiluminescent 98 Substrate (Pierce)).

    Article Title: Nobiletin alleviates brain injury in uremic mice and inhibits indoxyl sulfate-induced neurotoxicity in HT22 cells through the phosphatidylinositol 3-kinase/protein kinase B signaling pathway
    Article Snippet: The equipment for electrophoresis and transfer was obtained from Bio-Rad (Hercules, CA, USA). .. The membrane was then blocked using 5% nonfat milk at room temperature for 1 h and subsequently incubated overnight at 4°C with primary antibodies targeting α-tubulin (2144), Bcl2 (15071), poly ADP-ribose polymerase (PARP) (9532), PI3K (4292), pyruvate dehydrogenase kinase 1 (PDK1) (3062), Akt (4691), p-PI3K (17366), p-PDK1 (3438), p-Akt (4060), and Bax (5023) (1:1000 each, Cell Signaling Technology, Danvers, MA, USA). .. Afterward, the membrane was incubated with HRP-conjugated anti-rabbit Immunoglobulin G (IgG) secondary antibody (1:2000, A0208, Beyotime, Shanghai, China), and HRP-conjugated anti-mouse IgG secondary antibody (1:2000, A0216, Beyotime, Shanghai, China).

    Incubation:

    Article Title: Identification of signaling networks associated with lactate modulation of macrophages and dendritic cells
    Article Snippet: The membranes were subsequently blocked overnight at 4 °C in 10 mL of tris-buffered saline with 0.1% (v/v) Tween-20 (TBST), supplemented with 5% (w/v) bovine serum albumin (Roche). .. The membrane was incubated with each primary antibody targeting phosphorylated STAT1 (p-STAT1, Tyr701, D4A7, Cell signaling technology (CST) #7649), p-STAT2 (Tyr690, CST #4441), p-STAT3 (Ser727, CST #9134), p-SAPK/JNK (Thr183/Tyr185, 81E11, CST #4668), p-SAPK/JNK (Thr183/Tyr185, 98F2, CST #4671), p-NF-κB p65 (Ser536, 93H1, CST #3033), β-Catenin (Ser45, D2U8Y, CST #19807), p-Akt (Ser473, CST #9271), p-Akt (Thr308, D25E6, CST #13038), p-Akt (Ser473, D9E, CST #4060), Akt (pan) (C67E7, CST #4691), p-PTEN (Ser380, CST #9551), p-GSK-3β (Ser9, D85E12, CST #5558), p-c-Raf (Ser259, CST #9421), p-PDK1 (Ser241, C49H2, CST #3438), p-p44/42 MAPK (Erk1/2, Thr202/Tyr204, D13.14.4E, CST #4370), p-p38 MAPK (Thr180/Tyr182, D3F9, CST #4511), and β-actin (CST #4967). .. The membrane was then washed with TBST, and a secondary antibody was incubated for 1 h. The membrane was then washed with TBST followed by chemiluminescence detection using HRP substrate (SuperSignal West Pico/Femto Chemiluminescent 98 Substrate (Pierce)).

    Article Title: Nobiletin alleviates brain injury in uremic mice and inhibits indoxyl sulfate-induced neurotoxicity in HT22 cells through the phosphatidylinositol 3-kinase/protein kinase B signaling pathway
    Article Snippet: The equipment for electrophoresis and transfer was obtained from Bio-Rad (Hercules, CA, USA). .. The membrane was then blocked using 5% nonfat milk at room temperature for 1 h and subsequently incubated overnight at 4°C with primary antibodies targeting α-tubulin (2144), Bcl2 (15071), poly ADP-ribose polymerase (PARP) (9532), PI3K (4292), pyruvate dehydrogenase kinase 1 (PDK1) (3062), Akt (4691), p-PI3K (17366), p-PDK1 (3438), p-Akt (4060), and Bax (5023) (1:1000 each, Cell Signaling Technology, Danvers, MA, USA). .. Afterward, the membrane was incubated with HRP-conjugated anti-rabbit Immunoglobulin G (IgG) secondary antibody (1:2000, A0208, Beyotime, Shanghai, China), and HRP-conjugated anti-mouse IgG secondary antibody (1:2000, A0216, Beyotime, Shanghai, China).



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